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Behavioral methods for the functional assessment of hair cells in zebrafish
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《医学前沿(英文)》 2017年 第11卷 第2期 页码 178-190 doi: 10.1007/s11684-017-0507-x
Zebrafish is an emerging animal model for studies on auditory system. This model presents high comparability with humans, good accessibility to the hearing organ, and high throughput capacity. To better utilize this animal model, methodologies need to be used to quantify the hearing function of the zebrafish. Zebrafish displays a series of innate and robust behavior related to its auditory function. Here, we reviewed the advantage of using zebrafish in auditory research and then introduced three behavioral tests, as follows: the startle response, the vestibular-ocular reflex, and rheotaxis. These tests are discussed in terms of their physiological characteristics, up-to-date technical development, and apparatus description. Test limitation and areas to improve are also introduced. Finally, we revealed the feasibility of these applications in zebrafish behavioral assessment and their potential in the high-throughput screening on hearing-related genes and drugs.
关键词: zebrafish (Danio rerio) behavior auditory startle response vestibular-ocular reflex rheotaxis
Effects of modafinil on vestibular function during 24 hour sleep deprivation
ZHAN Hao, XIE Sujiang, JIA Hongbo, WEI Sihuang, JING Baisheng
《医学前沿(英文)》 2007年 第1卷 第2期 页码 226-229 doi: 10.1007/s11684-007-0044-0
关键词: VVOR modafinil Vestibular function vestibular functional Initially
A modified chronic ocular hypertension rat model for retinal ganglion cell neuroprotection
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《医学前沿(英文)》 2013年 第7卷 第3期 页码 367-377 doi: 10.1007/s11684-013-0266-2
This study aimed to modify a chronic ocular hypertension (OHT) rat model to screen for potential compounds to protect retinal ganglion cells (RGCs) from responding to increased intraocular pressure (IOP). A total of 266 rats were prepared and randomly grouped according to different time-points, namely, weeks 3, 8, 16, and 24. Rats were sedated and eye examination was performed to score as the corneal damage on a scale of 1 to 4. The OHT rat model was created via the injection of a hypertonic saline solution into the episcleral veins once weekly for two weeks. OHT was identified when the IOP at week 0 was≥6 mmHg than that at week -2 for the same eye. Viable RGCs were labeled by injecting 4% FluoroGold. Rats were sacrificed, and the eyes were enucleated and fixed. The fixed retinas were dissected to prepare flat whole-mounts. The viable RGCs were visualized and imaged. The IOP (meanβ±βSD) was calculated, and data were analyzed by the paired t-test and one-way ANOVA. The OHT model was created in 234 of 266 rats (87.97%), whereas 32 rats (12.03%) were removed from the study because of the absence of IOP elevation (11.28%) and/or corneal damage scores over 4 (0.75%). IOP was elevated by as much as 81.35% for 24 weeks. The average IOP was (16.68β±β0.98)βmmHg in non-OHT eyes (n = 234), but was (27.95±0.97)βmmHg in OHT eyes (n = 234). Viable RGCs in the OHT eyes were significantly decreased in a time-dependent manner by 29.41%, 38.24%, 55.32%, and 59.30% at weeks 3, 8, 16, and 24, respectively, as compared to viable RGCs in the non-OHT eyes (P<β0.05). The OHT model was successfully created in 88% of the rats. The IOP in the OHT eyes was elevated by approximately 81% for 24 weeks. The number of viable RGCs was decreased by 59% of the rats in a time-dependent manner. The modified OHT model may provide an effective and reliable method for screening drugs to protect RGCs from glaucoma.
关键词: chronic ocular hypertension intraocular pressure retinal ganglion cells neuroprotection glaucoma
Regeneration of hair cells in the mammalian vestibular system
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《医学前沿(英文)》 2016年 第10卷 第2期 页码 143-151 doi: 10.1007/s11684-016-0451-1
Hair cells regenerate throughout the lifetime of non-mammalian vertebrates, allowing these animals to recover from hearing and balance deficits. Such regeneration does not occur efficiently in humans and other mammals. Thus, balance deficits become permanent and is a common sensory disorder all over the world. Since Forge and Warchol discovered the limited spontaneous regeneration of vestibular hair cells after gentamicin-induced damage in mature mammals, significant efforts have been exerted to trace the origin of the limited vestibular regeneration in mammals after hair cell loss. Moreover, recently many strategies have been developed to promote the hair cell regeneration and subsequent functional recovery of the vestibular system, including manipulating the Wnt, Notch and Atoh1. This article provides an overview of the recent advances in hair cell regeneration in mammalian vestibular epithelia. Furthermore, this review highlights the current limitations of hair cell regeneration and provides the possible solutions to regenerate functional hair cells and to partially restore vestibular function.
ZHANG Kangkang, GU Enhua, LU Junjie
《医学前沿(英文)》 2008年 第2卷 第3期 页码 295-297 doi: 10.1007/s11684-008-0056-4
XIA Jiao, KONG Weijia, ZHU Yun, ZHOU Yan, ZHANG Yu, GUO Changkai
《医学前沿(英文)》 2008年 第2卷 第3期 页码 264-268 doi: 10.1007/s11684-008-0050-x
关键词: resistance infrared infrared videomicroscopy depressive after-hyperpolarization potential
Bang-hua YANG,Liang-fei HE,Lin LIN,Qian WANG
《信息与电子工程前沿(英文)》 2015年 第16卷 第6期 页码 486-496 doi: 10.1631/FITEE.1400299
关键词: Ocular artifacts Electroencephalogram (EEG) Electrooculogram (EOG) Brain-computer interface (BCI) Spatial constraint independent component analysis based recursive least squares (SCICA-RLS)
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《医学前沿(英文)》 2016年 第10卷 第4期 页码 465-472 doi: 10.1007/s11684-016-0482-7
Previous studies have demonstrated the effects of different afferent fibers on electroacupuncture (EA)-induced analgesia. However, contributions of functional receptors expressed on afferent fibers to the EA analgesia remain unclear. This study investigates the roles of acid-sensing ion channel 3 (ASIC3) and transient receptor potential vanilloid 1 (TRPV1) receptors in EA-induced segmental and systemic analgesia. Effects of EA at acupoint ST36 with different intensities on the C-fiber reflex and mechanical and thermal pain thresholds were measured among the ASIC3−/−, TRPV1−/−, and C57BL/6 mice. Compared with C57BL/6 mice, the ipsilateral inhibition of EA with 0.8 C-fiber threshold (0.8Tc) intensity on C-fiber reflex was markedly reduced in ASIC3−/− mice, whereas the bilateral inhibition of 1.0 and 2.0Tc EA was significantly decreased in TRPV1−/− mice. The segmental increase in pain thresholds induced by 0.3 mA EA was significantly reduced in ASIC3−/− mice, whereas the systemic enhancement of 1.0 mA EA was markedly decreased in TRPV1−/− mice. Thus, segmental analgesia of EA with lower intensity is partially mediated by ASIC3 receptor on Aβ-fiber, whereas systemic analgesia induced by EA with higher intensity is more likely induced by TRPV1 receptor on Ad- and C-fibers.
关键词: electroacupuncture analgesia ASIC3 TRPV1 C-fiber reflex
标题 作者 时间 类型 操作
Effects of modafinil on vestibular function during 24 hour sleep deprivation
ZHAN Hao, XIE Sujiang, JIA Hongbo, WEI Sihuang, JING Baisheng
期刊论文
A modified chronic ocular hypertension rat model for retinal ganglion cell neuroprotection
null
期刊论文
Setting up a heart rate alarm limit to decrease oculocardiac reflex during strabismus surgery in children
ZHANG Kangkang, GU Enhua, LU Junjie
期刊论文
Spontaneous firing properties of rat medial vestibular nucleus neurons in brain slices by infrared visual
XIA Jiao, KONG Weijia, ZHU Yun, ZHOU Yan, ZHANG Yu, GUO Changkai
期刊论文
Fast removal of ocular artifacts from electroencephalogram signals using spatial constraint independent
Bang-hua YANG,Liang-fei HE,Lin LIN,Qian WANG
期刊论文